The National Institutes of Health (NIH) is issuing a Notice of Funding Opportunity (NOFO) focused on advancing In Vivo Non-Invasive Optical Imaging Approaches for Biological Systems, with a funding opportunity number RFA-RM-24-012. This initiative aims to overcome the challenges posed by light scattering in biological tissues, which limits the depth and resolution of current optical imaging techniques. The NIH is encouraging the development of next-generation optical imaging technologies capable of providing high-resolution images across various biological scales, enhancing both diagnostic and research applications. Funding is available through a phased cooperative agreement mechanism (UG3/UH3), allowing for a maximum project period of three years, with initial support of two years followed by a potential one-year extension based on progress. Eligible applicants include higher education institutions, non-profits, and small businesses, with a total funding commitment of approximately $4.9 million anticipated. A key requirement for applicants is the Plan for Enhancing Diverse Perspectives (PEDP) to encourage inclusivity in research teams. Submissions must be made electronically through Grants.gov by February 7, 2025, encapsulating plans for innovative optical imaging technologies that can significantly improve penetration depth and resolution while addressing crucial biomedical challenges.