Institutions of higher education, public and private organizations, NGOs, and intergovernmental entities are all eligible to submit expressions of interest for the Addendum 01 - ROUTES. The program emphasizes partnerships with various organizations, encouraging collaboration across sectors while allowing applicants to submit multiple expressions of interest across different addenda. Funding availability will vary, and project execution details will be established during the design phase with selected partners.

The document addresses eligibility and application procedures for the Addendum 01 of USAID's initiative "Reducing Opportunities for Unlawful Transport of Endangered Species (ROUTES)." It clarifies that various organizations, including institutes of higher education, NGOs, and private sector partners, can submit expressions of interest. Applicants may include public, private, for-profit, and non-profit entities, emphasizing that collaborative efforts are encouraged, including participation from non-profit or intergovernmental entities within private sector projects. USAID will consider applications addressing any development sector in presence countries, but funding availability will depend on received expressions of interest. Selected partners will finalize project execution and funding details during the design phase. The document also specifies that organizations can apply to multiple Addenda or submit multiple expressions for different projects, underscoring the global scope of the initiative while maintaining that more applications do not necessarily improve chances of selection. The response provides a comprehensive overview of the application process, eligibility, and scope of opportunities for potential partners in biodiversity conservation efforts.