LICHEN RNA Analysis
ID: W9132T24Q0037Type: Solicitation
Overview

Buyer

DEPT OF DEFENSEDEPT OF THE ARMYW2R2 CONST ENGRG LABCHAMPAIGN, IL, 61822-1072, USA

NAICS

Research and Development in the Physical, Engineering, and Life Sciences (except Nanotechnology and Biotechnology) (541715)

PSC

NATURAL RESOURCES AND ENVIRONMENT R&D SERVICES; RECREATIONAL RESOURCES; BASIC RESEARCH (AH31)

Original Source

https://sam.gov/opp/31a15e880797440f81ef30da908ff39d/view
Timeline
    Description

    The U.S. Army Engineer Research and Development Center (ERDC) requires advanced genetic sequencing and analysis services for a project involving lichen samples. The main objective is to identify the genes expressed in lichen mycobiont and photobiont samples exposed to specific environmental conditions.

    The successful contractor will be responsible for transporting irreplaceable biological samples while maintaining RNA integrity. They will then perform RNA extraction, sequencing, and bioinformatic analysis using Illumina NovaSeq X technology, aligning the data with STAR and quantifying transcripts with salmon. The contractor must also participate in a kick-off meeting and provide regular updates as required.

    To be considered, applicants should have experience in RNA extraction and sequencing services, with a focus on dedicated use of a full 10B flow cell for 72 samples. The US Army Corps of Engineers (USACE) will evaluate applications based on their ability to meet the project's objectives and deadlines, with particular attention to the quality of the proposed services. Past performance, technical merit, and cost are key evaluation criteria.

    Interested parties should refer to the attached solicitation document for detailed requirements and expectations. Applications are due by 05 August 2024, with a revised deadline of 07 August 2024 for submissions. For clarification or questions, contact CERL Contract Team at CERL-CT-QUOTES@USACE.ARMY.MIL.

    Point(s) of Contact
    CERL Contract Team
    CERL-CT-QUOTES@USACE.ARMY.MIL
    Files
    Title
    Posted
    B08.01 Q&A Doc .docx
    The United States Army Corps of Engineers (USACE) requires genetic sequencing services, demanding dedicated use of a full 10B flow cell for 72 samples. This is a crucial aspect of their project, confirming the need for specialized sequencing services at a substantial scale.
    B08.01 QA Version 2.docx
    The US Army Corps of Engineers (USACE) seeks a contractor to process 72 biological samples using next-generation sequencing technology. The focus is on obtaining sequence data for pure cultures, specifically lichen biont tissues, through transcriptomics. The USACE aims to spread these samples across one full 10B flow cell of NovSeq X, generating approximately 50 million reads per sample. This requirement has been clarified in response to questions about the excessive read depth initially specified. The sequencing provider must ensure the samples' safe transportation while maintaining the cold chain, using dry ice for shipment. Contractors must propose their approach for the shipping method, detailing the expected transit time and dry ice requirements.
    B08.03 W9132T24Q0037 Amendment 0001.pdf
    The government seeks advanced RNA extraction, sequencing, and bioinformatic services from experienced contractors. The objective is to identify differentially expressed genes in lichen samples exposed to specific environmental stressors. The work statement entails transporting irreplaceable samples while maintaining RNA integrity, followed by high-quality RNA extraction and library preparation. Contractors will perform RNA sequencing using Illumina NovaSeq technology and then analyze the data, aligning it to provided genomes and conducting statistical analysis. Any issues and their potential solutions must be discussed with government researchers. The government will provide the biological samples and ensure contractor support. The kick-off meeting is required, with additional meetings as needed. Critical dates include a revised offer deadline of 07-Aug-2024 11:59 AM and a six-month contract completion deadline. The evaluation will consider the contractor's ability to meet these deadlines and the quality of their RNA extraction and sequencing services.
    W9132T24Q0037.pdf
    Through this RFP, the government seeks a contractor to provide services for "Lichen RNA Extraction, Sequencing, and Bioinformatic Analysis." The primary objective is to identify differentially expressed genes in lichen mycobiont and photobiont samples subjected to specific environmental stressors. The contractor must ensure the successful transportation of irreplaceable biological samples, preserving RNA integrity. They will then conduct RNA extraction, sequencing, and bioinformatic analysis according to specified methods and deliver high-quality sequencing data and reports. Key requirements include using Illumina NovaSeq X for RNA sequencing and performing bioinformatic analysis with STAR for alignment and salmon for transcript quantification. The government will provide the contractor with 72 biological samples and expects all services to be completed within six months. Critical dates include a submission deadline of 05 Aug 2024, and the project kick-off meeting is expected to occur within 30 working days of contract award. Evaluation of proposals will consider past performance, with technical merit and price also being key factors.
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    LICHEN RNA Analysis
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