CERC Generation of a Thiaminase-Knockout Zebrafish
ID: 140G0125Q0059Type: Solicitation
Overview

Buyer

INTERIOR, DEPARTMENT OF THEUS GEOLOGICAL SURVEYOFC OF ACQUSITION GRANTS-NATIONALRESTON, VA, 20192, USA

NAICS

Testing Laboratories and Services (541380)

PSC

SPECIAL STUDIES/ANALYSIS- ANIMAL/FISHERIES (B516)

Set Aside

Total Small Business Set-Aside (FAR 19.5) (SBA)

Original Source

https://sam.gov/opp/89a7f3d65862408095aa8d16c24ae79c/view
Timeline
    Description

    The U.S. Geological Survey (USGS) is seeking proposals from small businesses to develop a thiaminase-knockout zebrafish line, as part of a project funded by the Department of Defense's Strategic Environmental Research and Development Program (SERDP). The objective is to create a zebrafish model that targets a specific gene deletion, which will aid in understanding the impact of various stressors on thiaminase production among prey fish and the role of the microbiome. This initiative is structured into three phases: CRISPR design and sgRNA testing, somatic assessment through embryo injection, and germline transmission detection, with a total project duration of up to 24 months. Interested contractors must submit their proposals by February 7, 2025, and are encouraged to contact Kimberly Schneider at krschneider@usgs.gov for further information.

    Point(s) of Contact
    Schneider, Kimberly
    0000
    krschneider@usgs.gov
    Files
    Title
    Posted
    B08_Attachment_1__SOW_Zebrafish_Knockout_Line.docx
    The U.S. Geological Survey's Columbia Environmental Research Center seeks a contractor to develop a zebrafish knockout line targeting a specific thiaminase I gene deletion. This initiative supports research funded by the DoD's Strategic Environmental Research and Development Program (SERDP) on the role of various stressors in thiaminase production among prey fish and the influence of the microbiome on this activity. The project is structured into three phases: 1. **CRISPR Design and sgRNA Testing:** Identification and validation of single guide RNAs (sgRNAs) for effective gene editing. 2. **F0 Injection and Somatic Assessment:** Injection of embryos to induce somatic mutations and monitoring survival rates during rearing. 3. **Germline Transmission Detection and Line Delivery:** Screening F1 progeny for desired genetic edits and confirmation before preparing transgenic zebrafish for shipment. The work requires close collaboration with a designated USGS Technical Liaison and adheres to federal guidelines on animal welfare. The entire process is expected to last up to 24 months, culminating in the delivery of sequence-confirmed zebrafish for further research applications. This project illustrates the USGS's commitment to advancing ecological research methodologies through innovative genetic studies.
    Sol_140G0125Q0059.pdf
    The document outlines a Request for Proposal (RFP) from the U.S. Geological Survey (USGS) for the generation of a thiaminase-knockout zebrafish line, specifically aimed at small businesses. The RFP provides essential details such as the solicitation number (140G0125Q0059), the due date for offers (February 7, 2025), payment instructions, and the required documentation for submission, including pricing plans aligned with the project schedule and a work plan detailing technical approaches, timelines, and deliverables. Evaluation factors prioritize technical capability over cost, giving precedence to offers demonstrating relevant experience in similar projects, particularly involving gene deletions in model organisms. Additional instructions include compliance with various Federal Acquisition Regulation clauses and requirements for wage determination under applicable labor standards. The RFP emphasizes the necessity for interested contractors to maintain current registrations in the System for Award Management (SAM) and comply with electronic invoicing for payment. This solicitation aligns with federal objectives of supporting small businesses while promoting responsible labor practices and technical innovation in environmental research.
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    CERC Generation of a Thiaminase-Knockout Zebrafish
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