Intent to Sole Source - DNA Purification System
ID: 1204892Q0121Type: Special Notice
Overview

Buyer

AGRICULTURE, DEPARTMENT OFFOREST SERVICEPACIFIC NORTHWEST RESEARCH STATIONPORTLAND, OR, 97205, USA

NAICS

Testing Laboratories and Services (541380)

PSC

LABORATORY EQUIPMENT AND SUPPLIES (6640)
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    Description

    The Department of Agriculture's Forest Service, specifically the Pacific Northwest Research Station, intends to award a sole-source contract for the acquisition of a Thermo Scientific™ KingFisher™ Duo Prime Purification System. This specialized equipment is essential for projects focused on detecting invasive forest pathogens, which require high-quality DNA extraction from complex samples such as soil and water. The KingFisher system, utilizing advanced magnetic bead technology, allows for efficient processing of up to 96 samples simultaneously, addressing previous challenges faced with conventional DNA extractors. For further inquiries, interested parties can contact Nola Wisenor at nola.wisenor@usda.gov.

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    The Thermo Scientific KingFisher Duo Prime Purification System is designed to efficiently isolate DNA, RNA, and proteins from various starting materials, such as blood, tissue, and cell cultures, making it suitable for laboratories focused on nucleic acid purification and proteomics. This compact instrument utilizes automated magnetic bead technology for high recovery rates and reproducibility, and it has a small laboratory footprint. Key features include an optional bar-code reader for sample tracking and compatibility with KingFisher Pure isolation kits and customizable protocols via BindIt Software. The package includes a one-day SmartStart Orientation conducted by a Field Application Scientist to ensure swift setup and proper reagent selection tailored to laboratory needs. This document is part of federal RFPs aimed at acquiring equipment for scientific research, signifying a focus on enhancing laboratory capabilities and ensuring efficient workflow in nucleic acid handling and analysis.
    The USDA Forest Service's Pacific Northwest Research Station seeks approval for the sole-source purchase of a Thermo Scientific™ KingFisher™ Duo Prime Purification System with SmartStart, essential for BIL projects ERR09 and ERR10. These projects focus on developing technologies for detecting invasive forest pathogens across various climate zones and biological samples. The need for high-quality DNA extraction from complex samples, such as soil and water, is critical for the project's success. Previous attempts using conventional DNA extractors yielded unsatisfactory results. The KingFisher system, unique to ThermoFisher Scientific, utilizes magnetic bead technology, enabling efficient processing of up to 96 samples simultaneously. The purchase is urgent as delays jeopardize project deadlines and outcomes, underscoring the system's importance for advancing research in forest pathology and pathogen detection. This procurement aligns with federal guidelines for sole-source acquisitions due to the specialized nature of the required equipment and its proven efficacy in similar research settings.
    The USDA Forest Service's Pacific Northwest Research Station seeks approval to procure a Thermo Scientific™ KingFisher™ Duo Prime Purification System from Fisher Scientific™ for two research projects (ERR09 and ERR10) aimed at detecting invasive forest pathogens. The projects require high-quality DNA extraction from various biological samples, including soil and plant tissues. Previously attempted conventional extraction methods yielded unsatisfactory results, thus necessitating the unique capabilities of the KingFisher system, which utilizes automated magnetic bead technology for efficient DNA isolation. This system can process 96 samples simultaneously, enhancing productivity and supporting the urgent timeline of the projects. Fisher Scientific™ is the sole manufacturer of this equipment, and extensive attempts to identify alternative, comparable products failed, confirming its unique suitability for these research needs. The procurement is critical to ensure the project deadlines are met and the required high-quality DNA is obtained for further analysis and progression in scientific studies.
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