DNA Oligos for Amplicon Sequencing
ID: 12505B24Q0353Type: Combined Synopsis/Solicitation
Overview

Buyer

AGRICULTURE, DEPARTMENT OFAGRICULTURAL RESEARCH SERVICEUSDA ARS MWA AAO ACQ/PER PROPPEORIA, IL, 61604, USA

NAICS

Research and Development in Biotechnology (except Nanobiotechnology) (541714)

PSC

SPECIAL STUDIES/ANALYSIS- SCIENTIFIC DATA (B529)

Original Source

https://sam.gov/opp/b36b3c2d51034481900383df08dbb321/view
Timeline
    Description

    The Department of Agriculture, specifically the Agricultural Research Service (ARS), is seeking proposals for the procurement of DNA oligos for amplicon sequencing to support the genotyping of oysters at its facility in Kingston, Rhode Island. The procurement includes multiple line items, such as various 96-well plates and specific quantities of DNA oligos, which must meet stringent specifications for sequence authenticity and suitability for polymerase chain reaction (PCR). This initiative is crucial for advancing scientific research in marine aquaculture, reflecting the government's commitment to enhancing biotechnological capabilities. Interested contractors must submit their quotes electronically by 2:00 PM CDT on September 20, 2024, and can direct inquiries to Jeff Kathman at jeffery.kathman@usda.gov.

    Point(s) of Contact
    Jeff Kathman
    (515) 667-0841
    jeffery.kathman@usda.gov
    Files
    Title
    Posted
    Copy of oligo_plates_25nmol-1.xls
    The document outlines a series of DNA sequences associated with various WellPosition identifiers, primarily focusing on cvir (Candidatus Liberibacter asiaticus). Each identifier in the format represents a unique sequence for both forward (F) and reverse (R) primers, crucial for molecular biology applications such as polymerase chain reaction (PCR). The sequences include genetic codes vital for identifying specific genomic regions or organisms in research related to plant pathology and diagnostics. The structured listing categorizes primers into sets (A, B, C, etc.), suggesting a systematic approach to sequencing efforts for potential classification or studies in pathogen detection. The document serves as a foundational resource for laboratories engaged in genetic testing or agricultural biotechnology, reflecting the emphasis within federal government RFPs on promoting agricultural research and development initiatives.
    Copy of oligo_plates_25nmol-2.xls.xlsx
    The document consists of a comprehensive listing of sequence data pertaining to cvir, likely a virus or pathogen under surveillance or research within the context of federal grants or state and local Requests for Proposals (RFPs). Each entry is identified by a tagging system that comprises alphanumeric codes, followed by a defined genomic sequence. The sequences appear to be organized in a systematic format, suggesting that they may be instrumental for ongoing studies, perhaps in viral tracking, identification, or epidemiological research. The document's layout indicates that it serves as a resource for researchers or organizations interested in studying viral sequences for grant proposals or collaborations. The systematic listing captures a range of unique cvir identifiers and their corresponding sequences, presenting a tool for genetic analysis or application development. Overall, the document underscores governmental initiatives for scientific inquiry and the potential for biotechnological developments that could arise from basic research engagement in genomic studies. This consists of essential data required for advancing public health outcomes or response strategies in managing viral pathogens.
    oligo_plates_100nmol.xls
    This document presents a comprehensive list of genetic sequences organized by well position, with each sequence identified by its annotated label (e.g., A1, A2, etc.) and corresponding DNA sequences for various samples. The aim of this data appears to be for purposes related to biological research, possibly in the field of genetics or molecular biology. The sequences are iterated across different categories labeled A, B, C, etc., indicating variations in genetic compositions. The data provides supporting sequences featuring nucleotide compositions essential for scientific research, likely indicating the presence or absence of specific genes or genetic markers. These sequences are vital for understanding genetic diversity within tested samples, potentially impacting studies involving genetic analysis, biomedical applications, or ecological assessments. The structure of the document showcases well positions alongside their genetic sequences, allowing for easy reference. The detail and organization suggest a systematic approach to cataloging these genetic components, which is crucial in aligning with federal and state biological research requirements. This summary underscores the relevance of the document within government RFPs and federal grants aimed at advancing genetic research and development.
    oligo_tubes_100nmol.xls
    The document consists of a compilation of DNA sequences identified by specific names (GTseq_i7_i01 through GTseq_i7_i50), which likely represents a dataset related to genomic research or bioinformatics. Each sequence is a string of nucleotides designed for genetic analysis, possibly for applications like gene sequencing, therapy development, or genetic variations studies. While specific details on its usage are absent, the structured listing suggests an organized approach typical in genomic data management. Given the context of government RFPs and grants, this document may serve as a preliminary resource for entities seeking funding or support for research projects involving genetic analysis. The clarity of the sequences implies readiness for computational analysis, which could attract collaborations or proposals aligned with federal initiatives in healthcare, genetics, and bioengineering. Overall, the document encapsulates a vital dataset that lays the groundwork for advanced biological research and potential federal funding opportunities in these domains.
    Provisions and Clauses - DNA Oligos.pdf
    The document outlines the provisions and clauses associated with solicitation number 12505B24Q0353, detailing the terms and conditions for a federal contract. It incorporates various Federal Acquisition Regulation (FAR) clauses by reference significant for contractor compliance, particularly regarding commercial products and services. The clauses encompass federal mandates like compliance with the System for Award Management, restrictions on contracting from certain companies, requirements for small business preferences, and provisions for labor standards. Key points include the implementation of various laws and executive orders related to business ethics, equal opportunity, and procurement standards that support small businesses, particularly those owned by veterans and women. The document specifies the performance period, the locations for delivery, inspection, acceptance, and outlines the procedures for contract closeout. The primary purpose of this solicitation is to inform potential contractors of the terms under which the contract will be executed, ensuring they adhere to federal standards in their proposals and subsequent activities. The structure includes sections for contract clauses, provisions, evaluation criteria, and compliance measures, aiming to facilitate transparent and accountable government contracting processes.
    SF 1449 - 12505B24Q0353.pdf
    The document is a Request for Proposal (RFP) issued by the USDA Agricultural Research Service (ARS) for the procurement of laboratory supplies, specifically multiple lines of 96 Well Plate and DNA Oligos. The RFP number is ARS-12505B24Q0353, and it indicates a total of five line items with different quantities and specifications, indicating the need for both the products and shipping costs. The solicitation is open until September 9, 2024, with a performance period running from September 23, 2024, to October 22, 2024. The document includes detailed instructions for offer submission, including the requirement for the contractor to provide a signed document as well as information on payment methods and terms. Furthermore, the document adheres to regulations related to small business classifications and federal acquisition requirements. This RFP reflects the U.S. government's ongoing efforts to procure scientific equipment and supplies to support research initiatives within federal agencies, ensuring effective and compliant processes are followed in the acquisition of necessary materials.
    SF 30 12505B24Q0353 - 0001 Amendment.pdf
    This document is an amendment related to solicitation 12505B24Q0353 issued by the USDA ARS for procurement purposes. The primary objective of this amendment is to modify certain terms of the existing solicitation, particularly impacting small business set-asides. Key changes include the removal of the total small business set-aside, the elimination of specific clauses (AGAR Provision 452.219-70 and FAR Clause 52.219-6), and the addition of FAR Clause 52.219-4. Additionally, the deadline for submitting offers has been extended to September 20, 2024. The period of performance for the contract is noted as September 23, 2024, to October 22, 2024. All other terms and conditions of the solicitation remain unchanged, emphasizing the importance of acknowledging receipt of the amendment to avoid potential rejection of offers. This amendment reflects ongoing updates to ensure compliance and adjust provisions relevant to contractor eligibility. It serves to clarify and amend the solicitation process while maintaining the integrity of the procurement framework.
    Specifications_DNAoligos.pdf
    The USDA Agricultural Research Service seeks proposals for procurement of DNA oligos intended for amplicon sequencing to facilitate the genotyping of oysters at its Kingston, RI facility. Key specifications include provision of single-stranded DNA oligos verified for sequence authenticity through electrospray ionization mass spectrometry. Oligo yield must be measured by UV spectrophotometry, with deprotection and desalting purification procedures in place to ensure polymerase chain reaction suitability. Specific quantities of oligos are required across various formats: three 96-well plates with a target yield of 100 nmol, eight 96-well plates with a target yield of 25 nmol, and fifty individual tubes of 100 nmol oligos. Delivery of these items is mandated within 30 days post-purchase order, optimized for business hours and excluding federal and state holidays. The evaluation of proposals will focus on technical acceptance coupled with the lowest overall cost. This procurement serves to support scientific research relevant to marine aquaculture at a federal research facility.
    Updated Provisions and Clauses - DNA Oligos.pdf
    The document pertains to Solicitation 12505B24Q0353, detailing contract clauses and provisions for federal acquisitions. It outlines the incorporation of various clauses by reference, ensuring compliance with Federal Acquisition Regulations (FAR) relevant to commercial products and services. Key clauses include requirements on contractor ethics, prohibitions against certain telecommunications and hardware, and regulations aimed at promoting small business participation, such as HUBZone and SDVOSB programs. The solicitation also emphasizes conditions for the treatment of subcontractors and reporting obligations regarding employee rights and wage standards. Contract performance is specified to occur between September 23, 2024, and October 22, 2024, with the USDA-ARS National Cold Water Marine Aquaculture Center designated as the delivery and inspection location. Overall, this document primarily serves to formalize the legal and procedural framework guiding the solicitation process within federal contracting, promoting compliance, transparency, and inclusivity in procurement practices.
    Lifecycle
    Title
    Type
    DNA Oligos for Amplicon Sequencing
    Currently viewing
    Combined Synopsis/Solicitation
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