The U.S. Geological Survey (USGS) is seeking proposals for a Blanket Purchase Agreement (BPA) focused on the analysis of environmental DNA (eDNA) services, specifically related to macroinvertebrate communities and fecal source tracking in Johnson County, Kansas. The objective of this procurement is to assess stream health by analyzing eDNA samples collected from six locations, comparing current macroinvertebrate community compositions with historical data from 2003-2010, with a total of 24 samples for metabarcoding and 7 for qPCR analysis to be processed between February and April 2025. This BPA, effective from February 10, 2025, to February 9, 2030, emphasizes compliance with federal regulations and quality standards, requiring contractors to provide all necessary labor, equipment, and materials, while ensuring accountability through a quality assurance surveillance plan. Interested vendors can contact Jason Harris at jharris@usgs.gov or call 303-236-9316 for further details regarding the solicitation.