The Department of Defense (DoD) is seeking proposals for the development of antibiotic-loaded exosomes for topical application on wounds for prophylaxis or adjunct treatment indications. The objective is to address the increasing mortality rates caused by multidrug-resistant bacterial infections in wounded patients. The topic focuses on the use of exosomes, nanoscale vesicles optimized for intercellular communication, to transport antibiotics to target cells and promote wound healing. The Phase I of the project will involve exosome formulation with a broad or narrow spectrum antibiotic, with a focus on reproducibility, scalability, and stability. Phase II will include pre-clinical evaluation of the antibiotic-loaded exosome using in vitro and animal wound models. The deliverable for this phase will be an antibiotic-loaded exosome that demonstrates safety and efficacy. Phase III will involve in vivo and human research to obtain FDA approval, with potential funding opportunities from various programs. The use of a topical antibiotic for ESKAPE pathogens would have immediate utility in both military and civilian settings. The solicitation is closed, and more information can be found on the DoD SBIR website.