The Division of Global HIV and Tuberculosis, under the U.S. Centers for Disease Control and Prevention (CDC), has announced a Notice of Funding Opportunity (NOFO) for strengthening the organizational capacity of the Ministry of Health in the Kingdom of Eswatini. The grant, linked to the President’s Emergency Plan for AIDS Relief (PEPFAR), aims to enhance the response to HIV and tuberculosis (TB) while addressing related public health challenges. The application deadline is March 5, 2025, with expected awards ranging from $2 million for one to two cooperative agreements over a five-year performance period. The program seeks to bolster the Ministry of Health’s leadership, financial management, and workforce capabilities through evidence-based approaches. Key focus areas include improving governance structures, enhancing resource mobilization, and managing workforce performance. Eligible applicants include various institutions, from state and local governments to nonprofits and foreign entities, with priority given to local partners. Comprehensive reporting on progress towards specific outcomes will be required, along with a solid evaluation and data management plan. Applicants must demonstrate organizational capacity to manage funds and effectively implement health interventions. This funding opportunity highlights the U.S. government's commitment to sustaining progress against HIV/AIDS in Eswatini and strengthening overall health system resilience.