The Health Resources and Services Administration (HRSA) is inviting applications for funding under the Ryan White HIV/AIDS Program (RWHAP) as part of the Ending the HIV Epidemic initiative in the U.S. This funding opportunity, HRSA-25-063, aims to implement innovative strategies to reduce new HIV infections in jurisdictions with high HIV burdens, specifically targeting 48 counties, Washington, D.C., San Juan, Puerto Rico, and seven states with significant rural HIV challenges. The initiative emphasizes the importance of diagnosing all individuals with HIV, providing rapid and effective treatment, employing prevention strategies, and responding promptly to outbreaks, thereby enhancing the HIV care continuum. Approximately $175 million will be distributed across 47 cooperative agreements for a five-year period from March 1, 2025, to February 28, 2030, with applications due by October 22, 2024, and award notifications expected by February 1, 2025. For further inquiries, interested applicants can contact Yemisi Odusanya at YOdusanya@hrsa.gov.