The Notice of Funding Opportunity (NOFO) from the Department of Health and Human Services invites applications to support the Gabriella Miller Kids First Pediatric Research Program. This initiative emphasizes the exploration of genetic contributions to childhood cancers and structural birth defects through whole genome sequencing of pediatric cohorts. The program encourages diversity in study populations, particularly focusing on underrepresented racial and ethnic groups, to enhance the Kids First Data Resource Center with valuable genomic and clinical data. Submissions are open for various sequencing methods, including whole genome, exome, and transcriptome sequencing. The funding is designed for researchers to prepare cohorts for sequencing within a year without providing financial support for additional data collection or analyses. The NOFO outlines various eligibility criteria for applicants from academia, nonprofits, local governments, and international organizations. Key dates for application submissions begin February 13, 2024, with a final expiration on March 14, 2024. The overarching goal is to facilitate collaborative research that leads to advancements in understanding and treating childhood conditions, aligning with the needs for comprehensive data sharing as established by NIH policies. The NOFO emphasizes rigorous and responsible research practices while aiming to improve healthcare outcomes for affected children and families.