The Centers for Disease Control and Prevention (CDC) is forecasting a cooperative agreement opportunity aimed at strengthening laboratory systems that support the diagnosis, treatment, prevention, and management of HIV/AIDS in public health laboratories in Botswana under the President’s Emergency Plan for AIDS Relief (PEPFAR). The initiative seeks to enhance the capacity of the Ministry of Health to deliver quality laboratory services, focusing on improving laboratory referral networks, mentorship, continuous quality improvement, and biosecurity measures, while also expanding access to viral load services and early infant diagnosis. This funding is critical for bolstering laboratory capabilities in Botswana, with an anticipated total funding amount of $2,000,000 for the first year, and applications are expected to be submitted electronically by February 17, 2025. For further inquiries, interested parties can contact Emily Dale at (404) 639-8880 or via email at pepfarfoas@cdc.gov.