The U.S. Fish and Wildlife Service (FWS) intends to award a sole source firm fixed price order to The University of Alabama in Huntsville (UAH) for the continuation of a Biosurvey Study and eDNA efforts initiated in 2019. This project aims to enhance the understanding of subterranean biodiversity in Key Cave and its vicinity, focusing on identifying rare, threatened, and endangered species, including the Alabama Cavefish and Tuscumbia Cave Shrimp, through genetic analysis and resampling methodologies. The work is critical for the protection and conservation of these imperiled species, as UAH holds exclusive rights to the previously collected genetic data, ensuring consistency and continuity in the research. Interested parties may contact Chantal Bashizi at chantal_bashizi@fws.gov or call 703-358-1854 for further information, although this notice is not a request for competitive proposals.