B--DNA Extraction Cyanobacteria
ID: 140G0325Q0194Type: Solicitation
Overview

Buyer

INTERIOR, DEPARTMENT OF THEUS GEOLOGICAL SURVEYOFFICE OF ACQUISITON GRANTSSACRAMENTO, CA, 95819, USA

NAICS

Testing Laboratories and Services (541380)

PSC

SPECIAL STUDIES/ANALYSIS- CHEMICAL/BIOLOGICAL (B504)

Original Source

https://sam.gov/opp/14f9b94d3ea7471684abe821d29bfe4a/view
Timeline
    Description

    The U.S. Geological Survey (USGS) is seeking a contractor for DNA extraction and analysis services focused on samples collected from the San Francisco Bay Delta region. The project involves the collection of 100 samples from five monitoring stations between August 2025 and October 2026, with the contractor responsible for extracting DNA and performing quantitative PCR analyses on specific cyanotoxin biosynthesis genes. This initiative is crucial for advancing scientific understanding of aquatic ecosystems and monitoring environmental health in California. Interested vendors must submit their quotations by August 7, 2025, with final deliverables due by December 31, 2026; for further inquiries, contact Anthony Berry at aberry@usgs.gov.

    Point(s) of Contact
    Berry, Anthony
    (000) 000-0000
    aberry@usgs.gov
    Files
    Title
    Posted
    140G0325Q0194.pdf
    The U.S. Geological Survey (USGS) has issued Request for Quotations (RFQ) 140G0325Q0194 for DNA extraction and analysis services to evaluate samples collected from California's benthic habitats. This project is part of a study in the San Francisco Bay Delta, with sample collection slated to occur from July 2025 through October 2026. Contractors must provide a firm, fixed-price quotation, demonstrating their capability to perform specific DNA analyses using qPCR for designated genes. The contract’s period of performance extends until December 31, 2026. Interested vendors must follow detailed submission guidelines, including providing technical capability statements, compliance certifications, and requisite documentation. The RFQ outlines criteria for evaluating submissions, such as technical capability, past performance, and price. A clear emphasis is placed on the requirement for the contractor's laboratory leader to possess a Ph.D. in a relevant field and background in handling cyanobacteria samples. The document also covers invoicing terms, anti-malicious code measures, and contractor responsibilities, underscoring the government's commitment to maintaining quality and safety standards. The RFQ reflects the USGS's ongoing efforts to advance ecological research while ensuring compliance with federal acquisition regulations.
    Sol_140G0325Q0194_Amd_0001.pdf
    The document serves as an amendment to solicitation number 140G0325Q0194, issued by the U.S. Geological Survey (USGS) for the California Water Science Center (CAWSC). It extends the deadline for responses to August 7, 2025, at 5:00 AM PDT, while updating the Statement of Work (SOW) for analyzing DNA from samples collected during a study in the San Francisco Bay Delta. The study will assess water quality by examining various genes related to cyanotoxin biosynthesis from samples collected between August 2025 and October 2026. Contractors are required to extract DNA from samples and conduct quantitative Polymerase Chain Reaction (qPCR) analyses on specific genes. All operations need full documentation, with final data due by December 31, 2026. The amendment clarifies that contractor qualifications include possessing a Ph.D. in related fields and prior experience with cyanobacteria. The document emphasizes accountability, establishing clear deliverables and timelines to ensure quality and compliance with government standards. Overall, it confirms the government’s initiative in environmental monitoring and improving public health through scientific research and data collection.
    Sol_140G0325Q0194.pdf
    The U.S. Geological Survey (USGS) is issuing a Request for Quotation (RFQ) for DNA extraction and analysis services, specifically focusing on qPCR analysis of samples collected from benthic habitats in California. This RFQ (No. 140G0325Q0194) seeks quotations to be submitted by July 31, 2025, with a delivery deadline extending to December 31, 2026. The contractor will perform DNA extraction from 100 samples, utilizing various molecular methods to prepare sequencing libraries and execute analyses for specific genes associated with cyanobacterial ecology. Proposals must include a fixed-price quote, technical capability statements, compliance with FAR regulations, and relevant documentation. The contractor's capabilities must include substantial experience with cyanobacteria and the requisite analytical methods. The RFQ underscores the importance of compliance with federal requisites and highlights the necessity for proper documentation and technical oversight throughout the project duration. This effort demonstrates USGS's commitment to advancing scientific knowledge regarding aquatic ecosystems in California.
    SOW_140G0325Q0194_revised_0001.docx
    The U.S. Geological Survey's California Water Science Center requires a contractor for DNA analysis of samples collected from the San Francisco Bay Delta region. The study involves the collection of 100 samples from five monitoring stations between August 2025 and October 2026. The contractor will extract DNA from filtered samples and perform quantitative PCR analyses on specific cyanotoxin biosynthesis genes, including mcyE, anaC, sxtA, and the phycocyanin gene cpcB. Deliverables will consist of data files in various formats (XLS, CSV, TXT) due by December 31, 2026, along with accompanying summaries. The laboratory responsibilities encompass strict documentation of sample handling and bioinformatic analysis. Additionally, the laboratory must have a Ph.D.-qualified leader with relevant expertise, specifically in cyanobacterial ecology and molecular methods applicable to cyanotoxins in California. This RFP aligns with federal initiatives to monitor and analyze environmental health related to aquatic ecosystems.
    Lifecycle
    Title
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    DNA Extraction Cyanobacteria
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