Alligator Snapping Turtle eDNA Analysis from Filtered Water Samples
ID: 1132837Type: Sources Sought
Overview

Buyer

AGRICULTURE, DEPARTMENT OFFOREST SERVICEUSDA-FS, CSA EAST 8Atlanta, GA, 303092449, USA

NAICS

Testing Laboratories and Services (541380)

PSC

SPECIAL STUDIES/ANALYSIS- CHEMICAL/BIOLOGICAL (B504)

Set Aside

Total Small Business Set-Aside (FAR 19.5) (SBA)

Original Source

https://sam.gov/opp/f44eb033ad0b4ca5a4d097e46b6c336b/view
Timeline
    Description

    The US Department of Agriculture, Forest Service is seeking sources capable of performing environmental DNA (eDNA) analysis from filtered water samples to detect the presence of Alligator Snapping Turtles.

    The scope of work involves analyzing 135 water samples, collected from lakes and streams in Texas, using a specific method outlined by Spear et al. (2015) and Feist et al. (2018). The contractor must extract DNA from the samples and amplify it using a thermocycler profile. The process must ensure no cross-contamination and each sample must yield distinct results.

    Additionally, the contractor must employ a modified version of the testing methodology to validate the overall research study findings. Capability statements demonstrating the use of the specified testing methods and ensuring consistency with prior testing are crucial.

    The contract is expected to be a small business set-aside with an estimated value of $50,000 to $250,000. Laboratories capable of performing the specialized analysis are eligible to apply.

    The submission deadline is unspecified in the provided information. Interested parties should submit detailed capability statements via email to Laniece Nixon at laniece.nixon@usda.gov, with a courtesy copy to Keith Friot.

    Applications will be evaluated based on their ability to demonstrate a successful track record of performing the specific eDNA analysis using the required methods and equipment.

    For any clarification or further details, interested parties can contact Laniece Nixon or Keith Friot via email or phone. Nixon's email is laniece.nixon@usda.gov, and Friot's email and phone number are keith.d.friot@usda.gov and 9104751968, respectively.

    Point(s) of Contact
    Laniece Nixon
    laniece.nixon@usda.gov
    Keith Friot
    (910) 475-1968
    Keith.d.friot@usda.gov
    Files
    No associated files provided.
    Lifecycle
    Title
    Type
    Alligator Snapping Turtle eDNA Analysis from Filtered Water Samples
    Currently viewing
    Sources Sought
    Similar Opportunities
    Intent to Sole Source - DNA Purification System
    Active
    Agriculture, Department Of
    The Department of Agriculture's Forest Service, specifically the Pacific Northwest Research Station, intends to award a sole-source contract for the acquisition of a Thermo Scientific™ KingFisher™ Duo Prime Purification System. This specialized equipment is essential for projects focused on detecting invasive forest pathogens, which require high-quality DNA extraction from complex samples such as soil and water. The KingFisher system, utilizing advanced magnetic bead technology, allows for efficient processing of up to 96 samples simultaneously, addressing previous challenges faced with conventional DNA extractors. For further inquiries, interested parties can contact Nola Wisenor at nola.wisenor@usda.gov.
    High-Density Single Nucleotide Polymorphism Genotyping for Porcine
    Active
    Agriculture, Department Of
    The Department of Agriculture, specifically the Animal and Plant Health Inspection Service (APHIS), is soliciting quotes for high-density Single Nucleotide Polymorphism (SNP) genotyping services for feral swine, utilizing an Illumina Beadchip array or an equivalent technology. The contract, which is a firm fixed-price agreement for one year, aims to support the ongoing management of the National Feral Swine Damage Management Program, requiring a minimum of 60,000 autosomal loci per genotype assessment along with various sample extractions. This procurement is critical for generating data that will contribute to the government's genetic archive efforts, essential for wildlife management and research. Interested contractors must submit their proposals to Samantha Johnson by September 18, 2024, ensuring compliance with applicable Federal Acquisition Regulation (FAR) clauses and including necessary documentation and pricing schedules.
    Forest Service - SilverKing Barrier Assessment and Design
    Active
    Agriculture, Department Of
    The USDA Forest Service is seeking qualified contractors to conduct a barrier feasibility assessment and design for the re-introduction of the Paiute cutthroat trout in the Silver King gorge, spanning Alpine County, CA, and Washoe County, NV. The project requires a comprehensive evaluation of existing geological data, field data collection, topographic surveys, and the development of design plans that comply with permitting standards set by the Lahontan Regional Water Quality Control Board and the Army Corps. This initiative underscores the government's commitment to environmental conservation and the recovery of native species, with deliverables due by December 31, 2025. Interested parties should contact Lisa McFarland at lisa.mcfarland@usda.gov or Tung (Joe) Nguyen at tung.nguyen@usda.gov for further details.
    ANALYTICAL LAB SAMPLE-TRINITY RIVER
    Active
    Interior, Department Of The
    The Department of the Interior, specifically the Bureau of Reclamation, is seeking qualified contractors to provide analytical laboratory services for environmental assessments related to the Trinity River. The procurement involves evaluating up to 380 samples for hazardous materials, including asbestos and heavy metals, with a performance period from September 29, 2024, to March 28, 2026. This initiative underscores the government's commitment to environmental safety and regulatory compliance, ensuring that all analyses adhere to federal standards and methodologies. Interested bidders must submit their quotes by September 17, 2024, at 7 AM ET, and can contact Kelly Cook at kcook@usbr.gov for further information.
    Sequencing Supplies
    Active
    Agriculture, Department Of
    The Department of Agriculture, specifically the Agricultural Research Service, is seeking quotes for Sequencing Supplies under a Total Small Business Set-Aside contract. The procurement includes essential genomic analysis tools, specifically the TruSeq DNA Nano Low Throughput Library Prep Kit, MiSeq Reagent Kit v3, and IDT for Illumina – TruSeq DNA UD Indexes v2, which are critical for efficient genomic library preparation and sequencing. These supplies are vital for research and clinical applications, ensuring high data quality and throughput in genomic studies. Interested vendors must submit their quotes electronically to Joel Maas at joel.maas@usda.gov by 5:00 PM Eastern Time on September 16, 2024, and must be registered in the System for Award Management (SAM) to be eligible for award consideration.
    Red Spruce Tree Stand Exams Cherokee National Forest
    Active
    Agriculture, Department Of
    The Department of Agriculture, specifically the Forest Service, is seeking qualified contractors to conduct Red Spruce Tree Stand Exams across approximately 4,441 acres in the Cherokee National Forest, located in Cleveland, Tennessee. The project requires vendors to be proficient in Southern Appalachian tree identification and to implement the US Forest Service's Common Stand Exam protocols using designated software. This initiative is crucial for assessing forest health and managing natural resources effectively, ensuring compliance with ecological preservation standards. Interested parties should contact Jonathan Lewis at jonathan.lewis2@usda.gov or call 659-214-9618 for further details, with the contract performance period set from February 1, 2025, to January 31, 2026.
    Short-Read Next-Generation DNA Sequencing System
    Active
    Health And Human Services, Department Of
    The Department of Health and Human Services, specifically the National Institutes of Health (NIH), is seeking quotations for a Short-Read Next-Generation DNA Sequencing System to enhance its laboratory capabilities for rare variant discovery. The system must meet stringent requirements, including high accuracy (≥90%), compatibility with existing library formats, and the ability to detect variants down to 0.001%, along with specific physical dimensions and training for researchers. This procurement is crucial for advancing scientific research capabilities within the NIH and will be processed under Simplified Acquisition Procedures, with no set-aside for small businesses. Interested vendors should submit their quotes, including pricing and compliance with technical specifications, to Matt Lear at matt.lear@nih.gov by the specified deadline.
    Sequencing of RNA Samples - RFQ
    Active
    Agriculture, Department Of
    The Department of Agriculture, specifically the Agricultural Research Service, is soliciting proposals for the sequencing of RNA samples as outlined in Request for Quote (RFQ) number 12505B24Q0357. The procurement aims to obtain sequencing services for 33 RNA samples derived from extracellular vesicles related to piglet gastrointestinal health, which includes RNA quality testing, library preparation, and the generation of sequencing data, with deliverables such as quality reports and FASTQ files to be completed within 300 days post-award. This initiative is crucial for advancing agricultural research through specialized scientific services, and the contract will be awarded based on a Lowest Price, Technically Acceptable (LPTA) evaluation approach. Interested small businesses must submit their quotes electronically by September 16, 2024, to Aaron Dimeo at aaron.dimeo@usda.gov, ensuring compliance with federal regulations and active registration in the System for Award Management (SAM).
    Notice of Intent to Sole Source: Wildlife GPS collars & Transmitters
    Active
    Agriculture, Department Of
    The USDA Forest Service is seeking to sole-source procure 80 wildlife GPS collars and 30 vaginal implant transmitters from Advanced Telemetry Systems Inc. This specialized equipment is essential for tracking white-tailed deer, wild pigs, and coyotes, featuring advanced capabilities such as satellite communication, user-commandable release mechanisms, and sensors for monitoring birthing. The total estimated value of this procurement is $150,000, with responses due by September 16, 2024. Interested vendors may express their capability to meet the specifications, although this notice is not a request for competitive quotes, and responses will be used solely to determine if competitive procurement is warranted. For further inquiries, contact Julie Nawrozzada at juricka.nawrozzada@usda.gov.
    NWW Biological Services Trap and Transport
    Active
    Dept Of Defense
    The U.S. Army Corps of Engineers, Walla Walla District, is seeking interested businesses for a service contract titled "NWW Biological Services Trap and Transport." The contractor will be responsible for providing quality assurance and fish sampling functions for the USACE Juvenile Fish Biological Services and Transportation Program at Lower Granite and Little Goose dams, as well as supporting juvenile bypass system operations at Ice Harbor and McNary dams during the base year of 2025 and an option year in 2026. This contract is critical for ensuring the welfare of juvenile fish populations and compliance with environmental regulations, particularly under the Endangered Species Act. Interested parties must submit their capabilities and business size information to Melita S. Doyle via email by 3:00 PM local time on October 3, 2024, as this notice serves for market research and planning purposes only.