The Department of Health and Human Services (NIH) has issued a Notice of Funding Opportunity (NOFO), RFA-AI-25-027, to establish multidisciplinary Tuberculosis Research Units (TBRUs). The goal is to enhance understanding of Mycobacterium tuberculosis (Mtb)-host interactions, bacterial and host heterogeneity, and determinants relevant to Mtb establishment, dissemination, and disease progression. Each TBRU must include an Administrative Core, at least one Scientific Core, and a minimum of two to a maximum of four interdependent Research Projects, with at least one involving animal studies and one involving clinical studies. The NOFO details application instructions, eligibility, funding information (up to $1.5 million direct costs per year for five years, with $10 million in FY 2026 for 4-5 awards), and review criteria. Applications are due November 7, 2025, and must be submitted electronically via ASSIST or institutional system-to-system solutions. Foreign organizations are eligible but foreign subawards/subcontracts are not permitted.