The National Institutes of Health (NIH) has issued a Notice of Funding Opportunity (NOFO) under the National Institute of General Medical Sciences (NIGMS) for focused technology research and development via the R01 grant mechanism. The initiative aims to support projects that develop innovative technologies essential for advancing biomedical research. Eligible projects must already demonstrate proof-of-concept, targeting the creation and validation of working prototypes without addressing specific biological questions. Applications integrated with clinical trials are not permitted and must align with NIGMS's mission of fostering basic research that enhances understanding of biological processes. Key components include specific budget guidelines, eligibility criteria for applicants, and stringent application instructions to ensure compliance. Budgets are not capped but must reflect genuine needs, and the maximum project duration is five years. The review process emphasizes scientific merit and innovation while discouraging incremental technology advancements. Interested applicants are advised to understand the responsive content required for submission, ensuring their project aligns with the NOFO goals. This funding aligns with NIH priorities to develop technologies and methodologies that propel biomedical discoveries, solidifying research capacity across various institutions while fostering diversity and collaboration among scientists.