The U.S. Agency for International Development (USAID) is issuing a Notice of Funding Opportunity (NOFO) for the "USAID Lifeline for Health" program aimed at improving comprehensive HIV care and treatment in South Sudan. The initiative seeks applications from eligible organizations for a cooperative agreement to support the South Sudan Care and Treatment Activity over five years, with an estimated total funding of $30 million. The program targets key populations and high-risk groups to enhance access to HIV prevention, testing, care, and treatment services, particularly focusing on at-risk young men, women, and children across various counties. Eligible applicants include both local and international organizations, with preference for local entities. A minimum required cost share of $1.5 million or 5% of the total award is stipulated. The program will focus on developing local partner capacity while delivering comprehensive HIV services to reduce new infections and improve overall health outcomes, aligning with global health targets. Applicants must demonstrate their management capabilities, experience, and proposed technical approaches to meet the program’s aims. The deadline for inquiries is December 4, 2024, with submissions due by January 21, 2025. This initiative underscores USAID’s commitment to enhancing healthcare and reducing HIV-related stigma in South Sudan.