The National Eye Institute (NEI) is issuing a funding opportunity for secondary data analyses related to vision research through the Research Grant (R21) program. The initiative focuses on analyzing existing data from clinical trials and health care datasets, enhancing techniques for statistically testing hypotheses without collecting new data. The goal is to address major causes of blindness and improve understanding of visual system development and function. Eligible applicants include educational and nonprofit institutions, with a total project budget of up to $275,000 over two years. Applications must aim to produce significant scientific findings with at least two peer-reviewed manuscripts within the timeline. Notably, innovations in ocular imaging and data sharing methodologies are encouraged, and all research must align with NEI’s priorities. The application process involves strict adherence to guidelines, deadlines, and registration protocols via Grants.gov and eRA Commons. Emphasizing collaboration and thorough planning, this funding opportunity aims to foster impactful research in vision health, supporting advancements in statistical methods and health data utilization.