The Far Forward Biological Sequencing (FFBS) program, managed by JPM CBRN SOF within the DoD, aims to develop and field a biological identification system for Special Operations Forces. This system will identify known and unknown biological warfare agents, addressing current limitations in far-forward environments. The program will leverage existing technology transition agreements for automated DNA/RNA sample preparation and advanced bioinformatics. This Other Transaction Agreement (OTA) focuses on developing a prototype FFBS Increment 2 system during the Technology Maturation and Risk Reduction (TMRR) phase, with an option for the Engineering and Manufacturing Development (EMD) phase. The prototype, to achieve a TRL of 7 or better within 24 months, will feature automated sample preparation, a nanopore sequencing device, and bioinformatics software, operable by non-laboratory trained users in field environments. The document details extensive requirements across program management, systems engineering, testing (TMRR and EMD), and integrated product support, adhering to numerous military and DoD standards.

The Joint Program Executive Office for Chemical, Biological, Radiological, and Nuclear Defense (JPEO-CBRND) seeks pre-proposals for Far Forward Biological Sequencing (FFBS) Increment 2: Automated Sample Prep. The objective is to develop a system to identify numerous Biological Warfare Agents (BWAs) with a Technology Readiness Level (TRL) of seven or higher within 24 months. This system will include an automated sample and library preparation device compatible with nanopore sequencing, a custom computing element, and bioinformatics software, operable in far-forward field environments by non-laboratory trained users. The program aims to award an Other Transaction Agreement (OTA) for prototype development during the Technology Maturation and Risk Reduction (TMRR) phase, with an option for the Engineering and Manufacturing Development (EMD) phase. The deadline for pre-proposals is December 8, 2025.