The Centers for Disease Control and Prevention (CDC) is offering a cooperative agreement aimed at strengthening national laboratory and HIV epidemiologic monitoring capacities in the Kingdom of Eswatini under the PEPFAR initiative. The primary objective of this funding opportunity is to enhance the country's response to HIV, TB, and related public health threats by improving laboratory systems, diagnostic operations, and epidemic monitoring capabilities. This initiative is crucial for ensuring timely and accurate diagnosis and management of these diseases, thereby contributing to health security benchmarks in Eswatini. Interested applicants can reach out to the CDC at pepfarfoas@cdc.gov, with an anticipated funding amount of $5,000,000 for the first year, and applications are due by February 17, 2026, with awards expected to be announced by September 30, 2026.