The U.S. Fish and Wildlife Service (USFWS) intends to award a sole source contract to Illumina Inc. for the procurement of sequencing reagents, kits, and primers essential for environmental DNA (eDNA) analysis at the Lamar National Fish Hatchery and Northeast Fishery Center in Pennsylvania. The specific items required include the NextSeq 1000/2000 P1 and P2 XLEAP-SBS Reagent Kits, as well as Illumina DNA/RNA UD Indexes Sets A-D, which are critical for maintaining uniformity in lab operations and ensuring quality assurance in data generated from various aquatic invasive species detection projects. Interested parties may submit responses demonstrating the advantages of competition by April 7, 2025, to Lee Ann Riley at lee_riley@fws.gov, as this announcement serves as the official notice of procurement with no intention of compensating for information provided.